DON ELISA Test Kit 

Application:

This test kit can be used to detect Deoxynuvalenol in wheat, barley, corn, feed and feed ingredients samples qualitatively and quantitatively.

Technical specifications:

• Sensitivity: 5 ppb

Detection limit:

• Beer: 5 ppb
• Corn, peanut, wheat and other food crop, feed: 100 ppb
• Corn products such as DDGS and corn germ meal, spray corn, Corn gluten meal, finished feed based on the raw materials and concentrate materials, precision feed: 250ppb

Recovery rate:

• Beer: 90±10%
• Corn, peanut and other food crop, feed: 95±15%
• Corn products such as DDGS and corn germ meal, spray corn, Corn gluten meal, finished feed based on the raw materials and concentrate materials, precision feed: 95±15%
• Wheat, grist: 80±10%

Cross-reaction rate:

• Deoxynuvalenol: 100%
• 3-Acetyldeoxynivalenol: <1%
• Coefficient of variation:                <10%

Components:

• Micro-well strips: 12 strips with 8 removable wells each
• 6 x concentrated standards: 1ml each, 0 ppb (zero standard), 10 ppb, 20 ppb, 45 ppb, 135 ppb, 405 ppb
• Enzyme conjugate: 6ml
• Antibody working solution: 6ml
• Substrate A: 6ml
• Substrate B: 6ml
• Stop solution: 6ml
• Concentrated washing buffer (10x): 40ml
• Concentrated sample diluent (5x): 40ml

Materials required but not provided:

• Equipment: microtiter plate spectrophotometer (450 nm/630nm), vortex (oscillator optional), centrifuge (funnel optional), balance: 0.01g quantity sensitive, gaduated pipettes: 25 ml, polystyrene centrifuge tube: 50m(Triangle bottle with plug optional)
• Micropipettes: single-channel 10µl ~ 100µl, 100µl ~ 1000µl, eight-channel 300µl
• Reagents: Methanol, deionized water

Ordering Information: